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Overall, this work establishes pyoverdines as metallophores involved in La uptake, re-framing them from strictly iron-scavenging siderophores to dual-purpose metallophores with Ln-binding activity.
The pulp and paper industry produces large volumes of condensed kraft lignin, which is challenging to convert to single chemical products. For this purpose, tandem chemical depolymerization and bioconversion to a single atom-efficient product is a potentially promising strategy. In this study, we conducted copper-catal...
This study presents a systematic comparison of sugar catabolic pathways that enabled development of strains suited for the tradeoffs between rate and yield and indicates that these performance metrics can reduce the minimum selling price of muconate-derived adipic acid and greenhouse gas emissions.
Pro proteomics, metabolomics, and 13C-fluxomics are applied to quantitatively compare central carbon and energy metabolism in wild-type P. putida and a muconate-producing strain for improved bioproduction from renewable feedstocks.
Results indicate that catechol, not PCA, is the principal bottleneck in muconate production via the PCA decarboxylation route originally demonstrated by Draths et al., refining the understanding of pathway limitations and offering new strategies for improving rate, yield, and strain resilience in muconate bioproduction...
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