A sensitive assay for estimation of DM1 fortified with trastuzumab in human serum utilizing positive LC-MS/MS electrospray ionization and its application to clinical study.
AIM A sensitive LC-MS/MS method was developed and validated for the estimation of DM1 fortified with trastuzumab in human serum. MATERIALS AND METHODS Quantification was performed in positive mode with protein precipitation extraction using DM4 as an internal standard. Chromatographic separation was achieved using gr...