PCR–based assays for determining mating status in field–weathered Ceratitis capitata with enhanced precision across conventional, quantitative, and droplet digital platforms
Accurately determining the mating status of the agricultural fruit fly pest Ceratitis capitata, commonly known as Medfly, is essential for timely and effective eradication efforts. To overcome the limitations of subjective DAPI–based staining assessments of females captured in Jackson dry traps and Multilure liquid tra...