Cognate T-cell labeling by conventional and unconventional antigen-presenting cells in vivo using a modular SrtA-based toolkit
An enhanced Labeling Immune Partnerships by SorTagging Intercellular Contacts (LIPSTIC) platform utilizing the immunologically inert human CD58/CD2 receptor-ligand pair (SrtA-hCD58/G5-hCD2) is described to provide a robust tool for studying how APC identity influences Ag-specific T-cell responses.