The YTH domain was recently demonstrated as a promising tool for recognizing and regulating m6A-modified RNAs. However, the deficiency in binding affinity (micromolar range) and selectivity (off-target binding) for m6A of the wild-type YTH domain limit its sensitivity and accuracy for m6A recognition. Engineering strat...
Michael S. Addo, Elise D. Ficaretta, Yi-Bo Chang et al.· ACS Chemical Biology· 0 citations
Traditional methods for protein bioconjugation, such as those targeting canonical amino acid residues, often suffer from a lack of site-specificity, yielding heterogeneous conjugates. Genetic code expansion (GCE) technology offers an exciting alternative, enabling site-specific incorporation of noncanonical amino acids...
R. Sarkar, Manas Joshi, Abhishek Chatterjee· Angewandte Chemie· 0 citations
Over the past two decades, engineering efforts have yielded aminoacyl-tRNA synthetases (aaRSs) capable of charging diverse noncanonical amino acids (ncAAs). Here, we report an unexpected and exploitable activity of these engineered aaRSs: In the absence of their cognate tRNA, aaRSs can release the ncAA-AMP intermediate...
T. Yared, Elise D. Ficaretta, Lena A. Voss et al.· ACS Chemical Biology· 0 citations
This platform provides a powerful approach for engineering next-generation AAV vectors with customizable targeting profiles for diverse therapeutic applications and preserves AAV integrity and infectivity, and enables programmable retargeting of AAV tropism toward disease-relevant receptors.
Quan Pham, Jake Glicksman, Abhishek Chatterjee· Methods in molecular biology· 0 citations
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