Improved Peptide Recovery in Low-Input MS-Based Proteomics Using High-Recovery StageTip Desalting and Nonionic Detergent-Assisted Reconstitution.
Low-input mass spectrometry (MS)-based proteomics is vulnerable to peptide loss during final sample preparation. We evaluated whether ChocoTip desalting and decyl maltose neopentyl glycol (DMNG)-assisted reconstitution improve peptide recovery from HeLa digests corresponding to 10 ng of protein (∼50 cells). ChocoTip re...