Increasing Lipid Nanoparticle mRNA Copy Number Improves Transfection Potency.
Lipid nanoparticles (LNPs) are the leading platform for delivery of RNA-based therapeutics. There is considerable interest in optimizing the transfection potency of these systems. Standard formulation protocols involve mixing lipids in ethanol with a buffer containing mRNA at pH 4, followed by dialysis against a pH 7.4...