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Author

Aleš Štrancar

2 papers indexed here

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Open access Oct 2026

Development of Immobilized Restriction Enzyme Reactors on a Monolith Backbone for Streamlined Plasmid DNA Linearization.

High-quality, properly linearized plasmid DNA (pDNA) is a decisive upstream determinant of in vitro transcription (IVT) performance, impacting mRNA yield, integrity, and batch-to-batch consistency. We developed immobilized restriction enzyme reactors (IMERs) on monolithic supports and benchmarked EcoRI, NdeI, and HindI...

Maša Nabergoj, Špela Peršič Debenjak, Polona Skrt et al. · 0 citations
Open access Sep 2026

Enhancing Lentiviral Purification: Combining CIM QA Monolithic Columns With Design of Experiments Approach.

Use of lentiviral vectors (LVs) has been steadily increasing over recent years, culminating with a recent focus on novel in vivo therapies. Despite an increased focus on LV therapies, the methods for their purification have not yet been standardised. Additionally, no major focus has been given to the problem of heterog...

Luka Bevc, Ana Pavšič Rijavec, Valentina Novak et al. · 0 citations

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