Author

Alina Sieber

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Open access Jul 2026

Reprogramming a Protein Ligase for Genetic Code Expansion

It is demonstrated that chEpmA serves as a versatile dual-specificity platform: it efficiently charges tRNAs with the non-canonical backbone (R)-β-lysine, and a single substitution unlocks the scaffold for α-substrates, thereby enabling a broad spectrum of post-translational modifications previously inaccessible to gen...

Giovanni Gallo, Alina Sieber, Michael Hellwig et al. · bioRxiv · 0 citations