A CRISPR-Based Rapid Detection Assay for Crayfish Plague (Aphanomyces astaci) From Environmental Samples.
Crayfish plague, caused by Aphanomyces astaci (Aa), is an infectious disease invasive in Europe, where its rapid spread has resulted in sharp declines of native crayfish species. Monitoring currently relies on a highly sensitive, but costly and time-consuming qPCR approach. Here, we designed a simplified, rapid and cos...