Semi-Rational Design of Lignin Peroxidase for Enhanced Activity and Application in Wheat Straw Lignin Degradation
Lignin peroxidase (LiP) is a key bottleneck in wheat straw lignin degradation due to its limited catalytic efficiency. In this study, a lignin peroxidase from Phanerochaete chrysosporium (PcLiP; GenBank accession no. X15599.1) was engineered using a semirational design strategy. Seven candidate residues were identifi...