An-Ming Wang

Computational framework-guided engineering of microbial transglutaminase enables site-specific conjugation of Trastuzumab.

Microbial transglutaminase (MTGase) specifically catalyzes the conjugation between primary amine-containing molecules and glutamine residues, making it a promising enzymatic approach for producing homogeneous antibody-drug conjugates (ADCs). However, this strategy faces challenges associated with glycan-induced steric...