A Protocol for Multivariate Data Visualization and Pseudotime Modeling for Analysis of Disease Trajectories Detected by Mass Spectrometry Imaging.
Mass spectrometry imaging (MSI) has emerged as a powerful modality for spatially resolved molecular profiling of tumor and stromal compartments; however, computational frameworks for MSI data analysis lag significantly behind those developed for spatial transcriptomics, limiting its translational potential. Here, we in...