2026
A Golden Gate-Compatible CRISPR-Associated Transposon Tool for Multiplexed Bacterial Genome Editing.
An improved tool called pSPIN-GG and supporting protocols for simplified CAST-based genome engineering are presented and refinements support accelerated library construction, reduce assembly and screening burden, and expand the accessibility of CAST systems for multiplexed bacterial genome engineering.
Thea C T Irvine, Andrew M Bailey, Thomas E. Gorochowski
· Methods in molecular biology · 0 citations