Open access
Jul 2026
Top-Down versus Bottom-Up Proteomics in Highly Sensitive LC-MS-Based Profiling of Limited Samples
It is anticipated that TDP, enhanced by ultralow-flow PLOT chromatography columns coupled to MS, could be a supplementary or an alternative approach for limited-sample analysis, as it eliminates the need for protein digestion and minimizes sample cleanup, enabling rapid sample preparation while preserving proteoform in...
Yunfan Gao, Michal Greguš, James C. Kostas et al.
· Analytical Chemistry · 0 citations