Ansuman T. Satpathy

In vivo intercellular CRISPR screens using viral proximity barcoding reveal regulators of tumor-immune interactions

Systemic iron sequestration restricts the intratumoral CD8+ T cell landscape in pancreas cancer

Systemic iron sequestration occurs frequently in cancer due to inflammation-driven expression of the iron-regulatory hormone hepcidin. The impact of systemic iron availability on tumoral immunity is unclear. Here, we show that elevated serum hepcidin is associated with reduced survival and decreased intratumoral CD8+ T...

Cell cycle-coupled transcriptional network regulates human B cell fate bifurcation 2257411

A generalizable framework for assembling and testing GRNs in the context of immune cell fate decisions is established, and previously unreported feedback loops that link cell division to fate specification are revealed.

Genome-scale perturb-seq in primary human CD4+ T cells maps context-specific regulators of T cell programs and human immune traits

This study perturbed all expressed genes across 22 million primary human CD4+ T cells from four donors and developed a probe-based perturb-seq platform to measure the transcriptome effects in cells at rest and after stimulation, allowing it to map genes regulating immune pathways, including previously uncharacterized r...

Synthetic transcription factors designed by domain recombination enhance CAR T cell antitumor function.

It is demonstrated that reconfiguring existing protein domains may uncover non-evolved genes that program therapeutically relevant cell states that program therapeutically relevant cell states.

Mapping enhancer–gene regulatory interactions from single-cell data

A family of classification models, scE2G, is introduced that predict enhancer–gene regulatory interactions from single-cell datasets and enable mapping of these interactions across diverse cell types and tissues and will enable accurate mapping of enhancer–gene regulatory interactions across thousands of human cell typ...