Open access
Aug 2026
Directed Evolution of an Escherichia coli Secretor Strain Using the Curli Pathway
This work developed a Green Fluorescent Protein (GFP) secretion system by fusing GFP of the N-terminus sequence from the curli monomer protein while co-expressing the curli export machinery and found that secretion was sequence-specific, although no simple metric could predict success.
Anton Kan, S. Emani, Neel S. Joshi
· ACS Synthetic Biology · 0 citations