Author

Anujith Kumar

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Open access Jul 2026

Designing novel adenine clamp-CD73 mutants delineating surface expression and enzyme activity through in silico and in vitro engineering.

Overexpression of CD73-F417V/F500V mutant in CD73 null-HEKs indicated stable surface expression but complete loss of Ecto-nucleotidase activity, thus providing a tool to dissect activity-dependent and independent functions of NT5E.

G. Mohan, Anaswara S Padmam, Anujith Kumar et al. · Scientific Reports · 0 citations