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Atish Wagh

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Open access Aug 2026

Improved Purification of sgRNA and pegRNA by Use of a 5’-Terminal Dioctyloxytrityl (DOT) Protecting Group

DOT chemistry is analogous to “DMT-ON” approaches that are well known and widely used in the field, and is fully compatible with existing synthesis workflows, but dramatically improves HPLC purification relative to DMT-ON approaches by providing higher retention and thus better resolution to enable the purification of...

Atish Wagh, Jonathan K. Watts · 0 citations

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