Author

Atsushi Okuma

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Open access Jul 2026

A practical workflow for CAR screening in primary T cells using fitness-guided design and mRNA electroporation

One of the selected variants showed significantly improved cytotoxicity despite lower expression frequency and exhibited higher CD62L within CAR-positive cells, suggesting enhanced intrinsic function with a less differentiated phenotype.

Atsushi Okuma, Y. Ishida, S. Hisada · bioRxiv · 0 citations