Detection of potato virus Y resistance gene markers in the potato germоplasm of Kazakhstan
Abstract The objective of this study was to screen Kazakhstan-developed potato varieties for DNA markers linked to genes conferring extreme resistance to potato virus Y (PVY) and to evaluate their resistance to PVY infection. The object of the study was the biomaterial from 36 potato varieties provided by Kazakh Scientific Research Institute of Fruit and Vegetable Growing Limited Liability Partnership and Kostanay Scientific Research Institute of Agriculture Limited Liability Partnership. To determine the level of resistance to potato virus Y in the samples, plants were artificially infected with the virus in open ground and under controlled phytotron conditions. The assessment was carried out by visual observation of symptoms on inoculated potato plants, as by enzyme-linked immunosorbent assay. 38% of the varieties have shown extreme resistance to potato virus Y, 16% - Medium resistance (MR), 8% - Susceptible (S) and 41 % - High sensitivity (HS). The results obtained can be used in breeding programs to create virus-resistant potato varieties, as well as in fundamental research on the mechanisms of potato resistance to potato virus Y.