A false sense of circularity: hidden variability in RNase R enzymes confounds reliable circRNA validation
Circular RNAs (circRNAs) are an emerging class of RNA molecules involved in development and disease. They are generated through back-splicing from pre-mRNA. RNaseR treatment, which degrades linear RNA while preserving circRNAs, is widely used for circRNA validation and enrichment. This short report demonstrated differe...