Author

B. G. C.

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2026

Gene Silencing by CRISPR Interference in Fusobacterium nucleatum.

This chapter presents a step-by-step protocol for designing sgRNAs, constructing CRISPRi plasmids, transforming F. nucleatum ATCC 23726, and evaluating gene silencing phenotypes, using the nonessential gene ftsW, which encodes a protein required for peptidoglycan synthesis and cell division, as a model target.

Shiqi Xu, B. C., Kexin Tan et al. · Methods in molecular biology · 0 citations

2026

A Conditional Plasmid System for Markerless Gene Deletion in Genetically Recalcitrant Fusobacterium nucleatum subsp. animalis.

This chapter provides a detailed, step-by-step protocol for implementing a conditional plasmid system that enables efficient, markerless gene deletion in FNA strains and provides a powerful and adaptable tool for advancing genetic studies in this genetically recalcitrant subspecies.

B. G. C., Chenggang Wu · Methods in molecular biology · 0 citations