Bovine bone is a collagen-rich byproduct with potential for producing osteogenic peptides. A recombinant collagenase was heterologously expressed in Escherichia coliBL21(DE3), purified, and characterized. The enzyme showed a specific activity of 231.05 U/mg and hydrolyzed bovine bone collagen-containing matrix with a...
Yan-Ru An, Yu Wang, Yue Sun et al.· Journal of Agricultural and...· 0 citations
The burgeoning demand for efficient multienzyme cascades necessitates advanced scaffolds capable of precise spatial organization; however, constructing heterostructured multilayer frameworks (HMFs) remains a formidable challenge due to harsh solvothermal conditions and imprecise enzyme localization. Herein, a biocomp...
Bao-Zhu Zhao, Zhi-Fei Cheng, Jin Mao et al.· Analytical Chemistry· 0 citations
Foodborne diseases pose a severe threat to human health. As a critical meat-borne pathogen, Escherichia coli O157:H7 (E. coli O157:H7) can trigger severe illnesses such as hemorrhagic enteritis, threatening public safety and causing a huge economic impact on the meat industry. However, previous detection methods have s...
Hong-Zhou Chen, Wei-Chao Wu, Meng-Yu Li et al.· Foods· 0 citations
A novel biosensor employing dual-signal output was developed through the combination of efficient magnetic separation and dual-modal precise detection that can sensitively detect S. aureus in meat matrices, thus showing good application prospects in food matrices and further improving the reliability and specificity of...
