Click chemistry ligation-initiated bisulfite-free assay on nanobeads for highly sensitive detection of site-specific 5 mC.
5-Methylcytosine (5 mC) represents the most prevalent and functionally significant DNA modification in mammalian genomes. Aberrations in its status are intricately associated with the pathogenesis of various diseases, including cancer and neurodegenerative disorders. The ultrasensitive detection of low-abundance 5 mC i...