Open access
Aug 2026
The Characterization of AAV Capsid Individual VP-Specific Charge Heterogeneity Using a High-Throughput Reduced Denatured iCIEF–Western Method
An optimized, highly sensitive capillary-based Western method to measure the apparent isoelectric point (pI) and detect charge heterogeneity at the individual VP protein level under reduced denatured conditions is introduced.
Gangadhar Dhulipala, Kun Lu, N. Palackal et al.
· Biophysica · 0 citations