Chemical Rescue Serves as a Predictive Proxy for Glycosynthase Activity on Glycosidic Bonds via a Shared Glycosyl Oxocarbenium Transition State
It is demonstrated that chemical rescue—the azide-mediated restoration of hydrolytic activity in nucleophile-deficient mutants—serves as a predictive, high-throughput proxy for glycosynthase activity as well as a structural and thermodynamic rationale for using CR to select for transition-state-stabilizing mutations.