A Restriction-Free Cloning Approach for Molecular Engineering of Plasmids.
A restriction enzyme-free and ligation-free PCR-based protocol widely applicable to any circular vector that enables targeted mutagenesis of the chikungunya virus (CHIKV) genome, offering a fast, efficient, and reliable strategy for generating mutant constructs suitable for virological and molecular studies.
N. T. Cerf, C. Filomatori
· Methods in molecular biology · 0 citations