Open access
Sep 2026
Toward transgenic multicellular parasites: CRISPR-Cas12a germline editing of Schistosoma mansoni eggs as key to success
Lack of transgenesis methods impedes functional studies in platyhelminths. We present a substantially improved ribonucleic protein complex (RNP)–based genome editing approach for Schistosoma mansoni comparing Cas9 and Cas12a, the latter attained more efficient homology-directed insertion of a 5′-C6-PEG10–modified doubl...
Max F. Moescheid, Prapakorn Wisitpongpun, Vanessa Gross et al.
· Science Advances · 1 citation