Bacteriophage genomes contain large fractions of uncharacterized genes, motivating high-throughput functional screens to illuminate their roles in host interaction and cytotoxicity. Here, we performed a genome-wide overexpression assay for a Pseudomonas putida KT2440-infecting phage, Emajogi, and identified eight cytot...
Benjamin Diaz, Stephen Won, Meghana Padala et al.· bioRxiv· 0 citations
Insertion and excision of genomic islands (GIs), chromosomally-integrated mobile genetic elements (MGEs), are major sources of microbial genome plasticity and can impact gene expression and phenotype of the host organism. GI mobilization also influences microbial communities beyond the host organism as GI excision gene...
Lillian C. Lowrey, Josué A. Rodríguez-Ramos, A. Zimmerman et al.· bioRxiv· 0 citations
Overexpression experiments show that Psh protein is secreted by this restored T1SS to confer complete phage resistance, uncover a previously uncharacterized phage defense system in P. putida based on secretion-mediated receptor masking.
Benjamin Diaz, Stephen Won, James D. Jaryenneh et al.· bioRxiv· 0 citations
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