Chang-Chun Hao

Glycosylation reshapes coumarin recognition by β-lactoglobulin: Polar-contact-solvation trade-off revealed by multispectroscopy and molecular simulation.

Ligand glycosylation is often assumed to enhance protein binding by increasing hydrogen-bonding capacity, but its actual role in coumarin-β-lactoglobulin (β-LG) recognition has not been clearly resolved. Here, fraxin (FXN) and its aglycone fraxetin (FXT) were used as a paired model to examine how a glucose moiety regul...