ERO1A is a hypoxia-ER bridge in NSCLC: from pan-cancer identification to a dual-reporter visualization system.
A dual-reporter system, ERO1Ap2-miRFP670/5HRE-EGFP, driven by the ERO1A promoter and hypoxia response element (HRE), enables real-time, visual monitoring of the dynamic interplay between ER function and the tumor hypoxic microenvironment by simultaneously tracking ERO1A promoter activity and HIF-1α pathway activation.