Tumor tissue is a dynamic system governed by complex transcriptional kinetics. Although scRNA-seq has revolutionized cellular profiling, it captures static expression snapshots, lacking direct access to transcriptional dynamics. Here, we present Kinetics-seq, a time-resolved single-cell method that integrates an in viv...
Shan-Shan Zhang, Yi-Ling Xu, Rui Cheng et al.· Proceedings of the National...· 0 citations
Conventional analysis of extracellular vesicle (EV)-derived microRNAs relies on vesicle lysis and RNA extraction, which disrupt native vesicular structure and eliminate intravesicular molecular cargoes. Here, we report a rigid DNA tetrahedral nanoprobes-based logic-gated Förster resonance energy transfer (FRET) plat...
Tumor-derived extracellular vesicles (EVs) are promising liquid biopsy biomarkers, yet their clinical translation is hindered by the trade-off between enhancement and reproducibility of SERS-based detection. Here, we present a microfluidic SERS platform integrating plasmonic nanobowl arrays that simultaneously achieve...
Ruoke Wang, Ai-Peng Chen, Yu-Qing Wang et al.· Nano letters (Print)· 0 citations
The MIRACLE platform is developed, an integrated system combining microfluidic magneto-controllable reversible affinity capture with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for efficient, multiplexed detection of tumor-derived EV DNA mutations.
