Charlene Mandimutsira

Structural and Functional Consequences of Arg512 Mutations in Methyl-CpG-Binding Domain Protein 4.

Methyl-CpG-binding domain protein 4 (MBD4) is a base excision repair glycosylase that removes the thymine of a T:G mispair. If left unrepaired, these mismatches can cause C>T transitions. Human MBD4 uses an arginine finger, R468, to flip the mispaired thymine into its active site and to stabilize the orphaned guanine....