Structure-guided engineering of a gatekeeper residue enhances polylactic acid depolymerization by a cutinase from Papiliotrema nemorosa
Enzymatic recycling of polylactic acid (PLA) is constrained by limited enzyme–polymer interactions, particularly insufficient adsorption onto highly hydrophobic solid surfaces. In this study, a cutinase-like enzyme from Papiliotrema nemorosa was heterologously expressed and characterized. Structural analysis suggested...