Advances in the integration of isothermal amplification with CRISPR-Cas systems for point-of-care nucleic acid biosensing in viral, environmental, and food safety applications
This study aims to systematically examine the integration of isothermal amplification (IA) with CRISPR-Cas systems for point-of-care nucleic acid biosensing, elucidating the synergistic mechanisms that resolve the sensitivity–specificity tradeoff in molecular diagnostics. A comprehensive literature survey (2...