Hematological and Biochemistry reference ranges for Black marsh turtle (Siebenrockiella crassicollis)
The Black Marsh Turtle (Siebenrockiella crassicollis), a common freshwater species in Thailand, is currently listed as endangered. To support effective health assessment and conservation, this study established hematological and biochemical reference ranges for S. crassicollis and evaluated alterations in blood profiles associated with improper environmental conditions and illness. Blood samples from 56 turtles were analyzed, including clinically healthy individuals (32; 15 males and 17 females) and unhealthy individuals (24). The hematologic and biochemical parameters, including red blood cell indices, white blood cell counts and differentials, as well as blood chemistry values such as proteins, liver enzymes, metabolites, and minerals, were evaluated and summarized as baseline data. Among hematologic parameters, lymphocyte counts were significantly higher in males than in females. In contrast, females exhibited higher levels of cholesterol and phosphorus, while males showed higher activity of certain liver enzymes. Variations in some biochemical parameters were also observed and appeared to be associated with environmental factors, particularly differences in water quality among sampling sites. The results of this study provide important baseline information for improving the understanding of health status, clinical assessment, and appropriate husbandry of this protected species, ultimately contributing to more effective conservation strategies.