A growth-phase-dependent dual-dynamic regulation circuit is developed that gradually activates serA expression and progressively represses serB expression as cells enter the stationary phase, thereby reducing 2-HG accumulation and alleviating L-serine auxotrophy.
Yu Zhang, Jun Wu, Jinghua Yu et al.· The Innovation Life· 0 citations
Methanol is a promising renewable C1 feedstock for sustainable single‐cell protein (SCP) production. However, its inherent cytotoxicity and metabolic trade‐offs between cell growth and protein synthesis remain significant bottlenecks. Here, we established an “evolutionary‐rational” dual‐driven paradigm to construct a h...
Chong Xie, Cheng-Chao Zhu, Jun-Ze Liu et al.· Biotechnology Journal· 0 citations
This GRAS-certified platform establishes a highly efficient and economically viable route for the sustainable production of high-value SCP from non-food substrates, and validate a circular bioeconomy model by converting electrochemically synthesized 13C-labeled acetate into SCP.
Daphnetin is a clinically established coumarin, but its native biosynthetic pathway remains elusive. In this study, a 2-oxoglutarate-dependent dioxygenase (2OGD), AtS8H, was identified that hydroxylates umbelliferone (UMB) to daphnetin , enabling the design of an artificial biosynthetic pathway. However, the pathway ef...
Shunmin Ji, Chong Xie, Yanyan Wang et al.· ACS Synthetic Biology· 0 citations
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