2026
Methods for the Evasion of Restriction-Modification Systems During Genetic Engineering of Fusobacterium Species.
This chapter describes strategies to evade RM defenses and improve transformation efficiencies across diverse Fusobacterium lineages, employing a sequence-based "RM-silencing" method that has enabled successful delivery of replicative plasmids, linear recombination templates, and transposon cassettes into previously intractable Fusobacterium clinical isolates.
Martha A. Zepeda-Rivera, Elsa F. McMahon, Kaitlyn N. Lewis et al.
· Methods in molecular biology · 0 citations