A Cascade–Cas3-enabled method called TRIM3 is reported that generates large deletions by targeting a randomly integrated transposon, enabling facile generation of a genome-reduced mutant library and represents a new avenue for large-scale genome modifications and the development of improved bioprocessing hosts.
E. Fulk, R. M. Swart, Akira K Nakamura et al.· Nucleic Acids Research· 0 citations
This study presents a systematic comparison of sugar catabolic pathways that enabled development of strains suited for the tradeoffs between rate and yield and indicates that these performance metrics can reduce the minimum selling price of muconate-derived adipic acid and greenhouse gas emissions.
Dowan Kim, Torrey M Lind, Chen Ling et al.· bioRxiv· 1 citation
Efficient co-utilization of hexose and pentose sugars from lignocellulose is essential for microbial bioconversion, yet engineered catabolic pathways can be unstable or suboptimal in complex resource environments. Here, we use a Pseudomonas putida strain engineered to catabolize xylose and arabinose to examine how reso...
Sunghwa Woo, H. Lim, B. Norton-Baker et al.· Nature Communications· 0 citations
Results indicate that catechol, not PCA, is the principal bottleneck in muconate production via the PCA decarboxylation route originally demonstrated by Draths et al., refining the understanding of pathway limitations and offering new strategies for improving rate, yield, and strain resilience in muconate bioproduction...
Alissa C. Bleem, Tracy L. Hodges, Torrey M Lind et al.· bioRxiv· 2 citations
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