Open access
Aug 2026
Improving the efficiency of CRISPR/Cas9-induced multiplex editing in Brassica napus L.
Three endogenous promoters with high expression levels in the hypocotyls and callus are identified, to replace the 35S promoter of Cas9, and enhanced sgRNA (esgRNA) enhanced CRISPR/Cas9 editing efficiency by 52.1% compared to native sgRNA.
Huailin Li, Songyue Deng, Yu Yalun et al.
· Horticulture Research · 0 citations