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Chunyi Hu

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Open access Jul 2026

Saturated mutagenesis screen of M-MLV reverse transcriptase identifies variants enhancing prime editing efficiency

A compact prime editor is developed by deleting the RNase H domain of the MMLV reverse transcriptase and a saturated mutagenesis screen targeting two DNA interacting regions within the PE2ΔR-RT Fingers domain is conducted to identify novel mutations that enhance prime editor performance.

Hui Li, Yifan Wang, Chenxin Zhang et al. · 0 citations
Open access Jul 2026

Shared CRISPR arrays underpin type I-A/I-B coexistence.

A shared-array logic in which Cas6a/Cas6b-compatible processing routes a single pre-crRNA to multiple type I effectors providing a potential mechanism for subtype co-existence, a plausible explanation for array-less (or "orphan") interference modules, and an evolutionary bet-hedging strategy that prevents Acrs from shutting down immunity wholesale are uncovered.

Kunming Liu, Chunjiang Ren, Xinyue Liu et al. · 0 citations