Author

Cristina de la Encarnación

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Jul 2026

5' Dual-Overhang Short PAM-less dsDNA as Switchable Activators of Cas12a trans-Cleavage for Amplification-Free miRNA Detection.

The potential of terminally engineered PAM-less dsDNA as a structural handle for programming Cas12a activity is highlighted and useful insight is provided for the design of CRISPR-based biosensing strategies.

Xiaohui Wang, Shuxin Zhao, Jiaye Jiang et al. · ACS Sensors · 0 citations