Combinatorial Lid-Domain Engineering and Computational Design Enable Simultaneous Enhancement of Activity and Thermostability of D-Allulose 3-Epimerase.
d-Allulose is a promising low-calorie rare sweetener; however, its industrial production is limited by the insufficient catalytic activity and thermostability of d-allulose 3-epimerases (DAEases). In this study, a combinatorial engineering strategy integrating lid-domain modulation and computational design was develope...