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Author

Daniel J. Boesch

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Open access Sep 2026

Molecular basis of nick ligation in the nucleosome by DNA Ligase IIIα

Genomic DNA is packaged into chromatin through a fundamental repeating unit known as the nucleosome core particle. Chromatinized genomic DNA is constantly exposed to endogenous and exogenous stresses that result in DNA damage, which must be repaired to maintain genome stability. Single-strand breaks (SSBs) are a preval...

Daniel J. Boesch, Nadia I. Martin, Chantal A. Kontor et al. · 0 citations
Open access Aug 2026

Structural basis of end processing in the nucleosome by polynucleotide kinase phosphatase

Cryo-EM structures of PNKP engaged with non-ligatable SSBs at three unique positions within the nucleosome reveal that PNKP locally deforms nucleosomal DNA to reposition the SSBs into the kinase and phosphatase active sites, providing a structural basis for the efficient processing of SSBs throughout the nucleosome.

Daniel J. Boesch, Nadia I. Martin, John J. Evans et al. · 0 citations

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