Daniel K. Schwartz

Targeted mutagenesis of a thermophilic mevalonate kinase for efficient catalysis at lower temperatures.

Thermophile-derived enzymes are of interest for cell-free biocatalysis due to their intrinsic thermostability, which simplifies purification via heat treatment and correlates with enhanced activity retention under cell-free reaction conditions. However, thermal stability often comes at the expense of slower turnover ra...