Structure-function analysis of access engineering in an FMNH2-dependent monooxygenase for enhanced polyethylene surface oxidation.
Enzymatic functionalization of polyethylene is fundamentally limited by a backbone consisting entirely of inert CH bonds, and a structural framework for enzyme-mediated PE oxidation remains limited. Here we identify PA2355 (FMO), a soluble FMNH2-dependent monooxygenase from Pseudomonas aeruginosa that hydroxylates untr...