Dynamically Coupled Network-Guided Engineering of Amine Dehydrogenase With Cofactor Recycling for Efficient Biosynthesis of (R)-3-Aminobutanol.
(R)-3-Aminobutanol is a valuable chiral amino alcohol widely used in the pharmaceutical industry. The amine dehydrogenase (AmDH)-catalyzed synthesis with inorganic ammonia as the amine donor represents one of the most promising routes for its production. However, this approach is limited by the low catalytic efficiency...