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Open access Aug 2026

Intracellular screening of nanobodies reveals an intrabody that inhibits ITCH E3 ubiquitin ligase

Traditional in vitro screening of a nanobody library combined with an intracellular bioluminescence resonant energy transfer (BRET) proximity sensor approach for sequence down-selection can yield strong in vitro binders that also perform well as intrabodies, in this case capable of binding to and inhibiting the enzymat...

J. Wang, Victor L. Lam, E. Dunn et al. · 0 citations

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