Intracellular screening of nanobodies reveals an intrabody that inhibits ITCH E3 ubiquitin ligase
Traditional in vitro screening of a nanobody library combined with an intracellular bioluminescence resonant energy transfer (BRET) proximity sensor approach for sequence down-selection can yield strong in vitro binders that also perform well as intrabodies, in this case capable of binding to and inhibiting the enzymat...