Bridging the antiviral gap: A BAC-based non-infectious SARS-CoV-2 replicon reveals potent antisense gapmers
To facilitate studies of SARS-CoV-2 replication and antiviral drug discovery, we developed a non-infectious SARS-CoV-2 replicon, pBAC-SARS-CoV-2_S/eGFP_ΔE, based on a bacterial artificial chromosome vector. The viral genome was modified to ensure non-infectivity while allowing replication monitoring: the spike (S) prot...